Darkseid makes his first official appearance in Chapter Twenty-Two of the Injustice: Gods Among Us Comic on Apokolips. He is seen overseeing the torture of an unseen figure as his son Kalibak approaches him. Kalibak informs his father of Superman's worldwide ceasefire on Earth. Darkseid muses if Superman has begun to soften before Kalibak asks to take a war party to personally investigate. When Kalibak sees just who it is Darkseid is torturing, the lord of Apokolips confirms the man's identity before saying, "He made a mistake." When Kalibak asks if he can be killed, Darkseid chides his son, "Of course not. Who would come for him?"
He then allows Kalibak to go, bidding him to kill the Kryptonian and take the Earth. Darkseid grins to himself as he asks to be left alone with his prisoner: the Black Racer, death himself, as Darkseid resumes his torture.
In the Injustice Annual, Darkseid hires the bounty hunter Lobo to go to Earth and kill Superman in retaliation for the death of Kalibak at the Man of Steel's hands. However, Lobo returns some time later after a 'therapy' session with Harley Quinn, and brazenly challenges the Dread Lord to a battle.